The eagle biosciences homocysteine hplc assay kit makes it possible to determine homocysteine in an easy, fast and precise method. Our assay kits use a competitive elisa technique and each kit comes with the hcy precoated on a 96well microtiter plate. Homocysteine elisa the axis homocysteine enzyme immunoassay is intended for the quantitative determination of total homocysteine in plasma or serum. Pdf enzyme conversion immunoassay for determining total. Elisa kit suppliers from china shanghai korain biotech co. The elisa enzymelinked immunosorbent assay is a widely used application for detecting and quantifying proteins and antigens from various samples. Buy hcy elisa kit, human hcy homocysteine elisa kit mbs766119 product datasheet at. Human homocysteine elisa kit allows for the in vitro quantitative determination of homocysteine concentrations in serum, plasma, tissue homogenates and cell culture supernates and other biological fluids. Elisa and matched antibody pair kits cell and tissue imaging tools cellular and biochemical assays. Covid 19 xpressbio is working diligently to ensure we keep our employees and their families safe, while maintaining our business continuity. For the quantitative determination of endogenic human homocysteine hcy.
Enzymatic homocysteine 3 reagent diazyme laboratories. Kt60099 intended use this hcy elisa kit is intended for laboratory research use only and is not for use in diagnostic or therapeutic procedures. Homocysteine elisa kit lsf10591 is a 96well enzymelinked immunosorbent assay for the quantitative detection of all species homocysteine in plasma, serum. Increased severity of diabetic retinopathy was found to correlate with an increase in the serum levels of. The kit has a detection sensitivity limit of 10 ngml homocysteinebsa. This immunoassay kit allows for the in vitro quantitative determination of general homocysteine concentrations in serum, plasma, tissue homogenates, cell culture supernates and other biological fluids. This elisa kit is used for quantitative measurement of homocysteine in serum, plasma, tissue homogenates and other biological fluids. It is a homologue of the amino acid cysteine, differing by an additional methylene bridge ch2. Detection of other special sample please contact our technical support. When stored and handled as directed, reagents are stable until the expiration date. Creative diagnostics provides homocysteine antibodies and elisa kits for science research use, feel free to contact us. Elabscience specializes in the selfdeveloped homocysteine hcy colorimetric assay kit enzyme circulation method, strict quality control, fast delivery, ensure your experiment needs. This package insert must be read in its entirety before using this product. Eh4011 detection method competitive elisa, coated with antigen application hcy elisa kit allows for the in vitro quantitative determination of hcy concentrations in plasma, tissue homogenates and other biological fluids.
All species homocysteine elisa kit sandwich elisa user manual catalog no. Faulty homocysteine recycling in diabetic retinopathy. Final absorbance was measured at 450 nm using an elisa plate reader. Total homocysteine thcy plasma abbott imx homocysteine hcy. Effect of seabuckthorn seed oil in reducing cardiovascular risk factors.
Product name human hcy homocysteine elisa kit catalogue no. Principle of the assay this experiment use doublesandwich elisa technique and the elisa kit provided is typical. K531100 homocysteine assay kit fluorometric biovision. Homocysteine elisa kit okeh02557 quantitative competitive. Homocysteine assay kit fluorometric k531 biovision, inc. The eagle biosciences homocysteine hplc assay kit is intended for the quantitative determination of homocysteine in plasma and serum. Increased serum level of homocysteine correlates with.
Homocysteine elisa kits the elisa enzymelinked immunosorbent assay is a widely used application for detecting and quantifying proteins and antigens from various samples. Involvement of homocysteine, homocysteine thiolactone, and. Lsf10591 it is important that you read this entire manual carefully before starting your experiment. It is a homologue of the naturallyoccurring amino acid cysteine, differing in that its sidechain contains an additional methylene ch2 group before the thiol sh group. Each column is can be utilized for approximately 500 samples depending on column care and sample type. Human homocysteine elisa kit can be provided from creative diagnostics. Measurement of homocysteine level the concentration of hcy in the blood and retinas of humans and different animal models of diabetes, representing type 1 and type 2 diabetes stztreated mice, dbdb mice and stztreated ratswas determined using an hcy enzyme linked immunosorbent assay elisa kit from cell.
Human homocysteine elisa kit, human homocysteic acid elisa kit, human hcy elisa kit, human homocysteine acid elisa kit intended use this immunoassay kit allows for the in vitro quantitative determination of homocysteine, homocysteine concentrations in serum, plasma, tissue homogenates and cell culture supernates and other biological fluids. Competitive kit in a competitive elisa assay, sample antigen and labeled antigen compete for capture antibody binding. Targetspecific elisa kits are available from a variety of manufacturers and can help streamline your immunodetection experiments. Csbe814h for the quantitative determination of endogenic human homocysteine hcy concentrations in serum, plasma, urine, tissue homogenates. Hcy homocysteine elisa kit from elisa genie is a precoated immunoassay with a sensitivity of 4. Sandwich kit the sample with an unknown amount of antigen is immobilized on a precoated plate either nonspecifically via adsorption to the surface or specifically via capture by another antibody specific to the same antigen. The kit has a detection sensitivity limit of 10 ngml homocysteine bsa.
Homocysteine assay kit fluorometric ab228559 allows for quantification of total homocysteine in biological fluids such as plasma and serum. Our homocysteine elisa is designed for detection and quantitation of homocysteine in plasma, serum, lysates, or biological fluid. Biovisions homocysteine assay kit allows for quantification of total homocysteine in biological fluids such as plasma and serum. Mouse homocysteine elisa kit competitive eia user manual catalog no. Validated in wb, ip, elisa, ihc, dot and tested in mouse, human. The loss rate of this kit is less than 10% within the expiration date under appropriate storage conditions. Mouse homocysteine hcy elisa kit mybiosource mbs260152. Hcyhomocysteine elisa kit from elisa genie is a precoated immunoassay with a sensitivity of. The homocysteine hplc assay kit is for research use only and should not be used for diagnostic procedures. Mouse homocysteine hcy elisa kit this product is suitable for in vitro quantitative detection of mouse serum, plasma or cell culture supernatant and organizations in the natural and recombinant hcy concentration. Elisa kits and reagents for therapeutic mabs, biosimilars, and proteins. The eagle biosciences sadenosyll homocysteine sah elisa assay kit is for research use only and not to be used in clinical, therapeutic or diagnostic procedures. Correlation of tsh with homocysteine and lipid profile in hypothyroidism a malik, et al,j liaquat uni med health sci,2018.
Arg80875 human homocysteine hcy elisa kit is an enzyme immunoassay kit for the quantification of human homocysteine hcy in serum and plasma edta. The homocysteine hplc assay kit is for the quantitative determination of homocysteine in plasma and serum and. If you have problems technical service contact information. Rat homocysteine assay kit fluorescence mouse homocysteine assay kit. Homocysteine elisa kit alpha diagnostic international. Homocysteine is an amino acid intermediate that is synthesized from methionine via sadenosylmethionine sam and sadenosylhomocysteine sah. The refe introduction homocysteine is a thiolcontaining amino acid produced by the intracellular demethylation of methionine. Gbioscienses immunotag mouse hcy homocysteine elisa kit quantity. Mouse homocysteine hcy elisa kit employs a twosite sandwich elisa to quantitate hcy in samples. A competitive elisa kit for the quantitative measurement of homocysteine in serum, plasma, culture supernatants and other biological fluids. This assay is a competitive elisa where samples and an anti homocysteine antibody are added to a plate coated with a homocysteine conjugate. Standards and samples are pipetted into the wells and any hcy present is bound by the immobilized antibody. The architect homocysteine reagent kit must be stored at 28c in an upright position and may be used immediately after removal from 28c storage. Homocysteine is a thiolcontaining amino acid produced by the intracellular demethylation of methionine.
Test principle the microtiter plate provided in human homocysteine elisa kit has been precoated with an antibody specific to homocysteine. An antibody specific for hcy has been precoated onto a microplate. Gbioscienses immunotag mouse hcy homocysteine elisa kit. This assay is a competitive elisa where samples and an antihomocysteine antibody are added to a.
Homocysteine assay kit allows for quantification of total homocysteine in biological fluids such as plasma and serum. Minor changes may occur to the detection range, sensitivity, and. Before shipping, each kit is assembled and tested to ensure that it meets specifications before shipping. The homocysteine hplc column can be used with eagle biosciences homocysteine hplc assay kit hcy31h100. The diazyme homocysteine enzymatic a ssay kit is intended for the in vitro quantitative determination of total l homocysteine in serum or plasma. Total homocysteine thcy plasma abbott imx homocysteine. Rat homocysteine hcy elisa kit employs a twosite sandwich elisa to quantitate hcy in samples. Buy homocysteine elisa kit, human homocysteine elisa kit mbs7252797 product datasheet at. Homocysteine inducible, endoplasmic reticulum stressinducible, ubiquitinlike domain member 1 elisa kits homogentisate 1,2dioxygenase elisa kits homolog of yeast recqmediated genome instability 1 elisa kits. Lsf36528 it is important that you read this entire manual carefully before starting your experiment. The homocysteine hplc assay kit includes all reagents for preparation and separation of the samples with exception of the columns ic2801rp and the controls ic2801ko. All species homocysteine quant elisa kit sandwich lsbio. Elisa kits and reagents for infectious disease and vaccine research. Bioassay technology laboratory bt lab is a brand of shanghai korain biotech co ltd specialized in lab supplies of elisa kit, antibodies, protein for life science research.
For this assay, 300 ll of medium bww containing 20 9 106 cells from the high and lowdensity regions of percoll gradients were. The eagle biosciences sadenosyll homocysteine sah elisa assay kit is intended for the quantitative determination of sadenosyll homocysteine sah in serum or plasma. Despite a stay at home advisory being put in place in maryland, usa, we are deemed an essential business, and our manufacturing and distribution teams continue to be fully operational. A longitudinal controlled trial on hypertensive subjects. Maxdiscovery homocysteine enzymatic assay kit specs. Homocysteine hcy is a thiolcontaining amino acid produced by the intracellular demethylation of methionine.
Our homocysteine elisa is designed for detection and quantitation of homocysteine in plasma, serum, lysates, or biological fluid samples. Homocysteine is a chemical compound with the formula hsch2ch2chnh2co2h. Homocysteine elisa kit is intended for the quantitative determination of total l homocysteine in plasma or serum. Statistical analysis was used to assess the correlations between the study variables. Elevated levels of homocysteine can indicate a risk for numerous diseases, including vascular and heart disease. To avoid evaporation of the reagents, the reagent pack kit should not be kept open in the abbott imx analyzer when a run is finished. To meet every single need of the customer we aim to provide high quality at best price and service with our professional staff. The axis homocysteine enzyme immunoassay is intended for the. Biomatik elisa kits are not typically preassembled as finished products due to limited shelf life. Pdf diabetic retinopathy dr is the most common cause of blindness in people under the age of 65. Standards preparation standards corresponding to 0. Maxdiscovery homocysteine enzymatic assay kit bioo scientific. Correlation of serum lipids, lipoproteins and homocysteine levels in hypothyroid patients of northwest india kuldip singh, et al,international journal of scientific research,2018. Hcy elisa kit human hcy homocysteine elisa kit mybiosource.
Aviva systems biology homocysteine kit human okeh02557 is based on standard sandwich enzymelinked immunosorbent assay technology. Homocystein elisa,homocystein,stroke, heart attack, homocystein elisa, homocystein. Human alpha 1 antitrypsin elisa kit serpina1 ab108798 abcam. The assay is based on the reduction of homocysteine disulfides to free homocysteine, which is cleaved by a homocysteine selective enzyme, generating an intermediate product. Homocysteine elisa kit cambridge bioscience limited. Maxdiscovery homocysteine enzymatic assay kit bioo. It is a sandwich assay which can detect homocysteine down to 0. Homocysteine hplc assay column eagle bio hplc assay column. The assay is based on the reduction of homocysteine disulfides to free homocysteine, which is cleaved by a homocysteine selective enzyme. Hcy is exported into plasma where it circulates mostly in its oxidized forms bound to plasma proteins. After removing any unbound substances, hrpconjugated mouse. For additional information on system and assay technology, refer to the. Technical manual rat homocysteine hcy elisa kit catalogue.
After removing any unbound substances, hrpconjugated rat. The maxdiscovery homocysteine enzymatic assay kit has the capacity for 48 determinations or testing of 24 samples in duplicate assuming 12 wells for standards. The antibody shop, the marketplace with over a million primary and secondary antibodies, elisa kits, qpcr kits, ria kits, assay kits and reagents. Samples and biotin labeling antibody are added into elisa plate wells and washed out with pbs or tbs. The stability of the mouse homocysteine elisa kit is determined by the loss rate of activity.
User manual competitive eia kit mouse homocysteine. The assay can assist in diagnosis and treatment of patients suspected of having hyperhomocysteinemia and homocystinuria. Abclonal provide all the timesavings, convenience and performance of a highend, commercial elisa kit for the competitive price. For the quantitative determination of endogenic human homocysteine hcy concentrations in. Our homocysteine elisa is designed for detection and quantitation of homocysteine in plasma, serum. Elisa kit for the detection of homocysteine in the research laboratory the antibodyshop, antibodies, elisa kits, reagents. Levels of homocysteine were measured in the retinal homogenate 15. The shelf life is 12 months when the kit is properly stored. General homocysteine elisa kit hcy rk00671 suitable for serum, plasma, cell culture supernatants and urine in general.
Smaller amounts of reduced homocysteine and disulfide homocystine hcysshcy are present. Description and principle the elisa genie hcy elisa kit assay can assay for the specific analyte in the. The sample and conjugate compete for antibody binding, which. General homocysteine elisa kit hcygeneral elisa kits. Human hcyhomocysteine elisa kit wuhan fine biotech co. Dec, 2019 the detection was carried out with reference to the instructions of the corresponding kits and instruments. Send me a copy of this email i agree to the terms and conditions. The stop solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. The precoated antibody is mouse hcy monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. The architect homocysteine reagent kit may be stored on board the architect i system for a maximum of 30. Elisa kit for the detection of homocysteine in the research.
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